EFFECT OF LACTUSAN ON INTESTINAL FUNCTION

PURPOSE OF THE RESEARCH

To determine the presence or absence of therapeutic and prophylactic properties of dairy products enriched with lactulose, produced according to the relevant technical documentation.

OBJECT OF THE RESEARCH

Kefir with the addition of lactulose "Lactusan" was chosen as the object of research. At the same time, the researchers assumed that the results of the study of this object can be transferred to other dairy products enriched with lactulose.

It is well known that kefir itself is a means of improving intestinal functions. Therefore, in order to determine the effectiveness of kefir enriched with lactulose, it was necessary to compare it with the effect of ordinary kefir produced using traditional technology.

Kefir enriched with lactulose was prepared at VNIMI using the following technology: first, kefir was prepared using traditional technology, then lactulose syrup "Lactusan" was added. The homogeneous mixture was poured into 200 ml molds and hermetically sealed with aluminum foil. Control samples were prepared in the same way, only without the addition of lactulose.

RESEARCH METHOD

The tests were conducted with two groups of children, one of which is considered the control (receives kefir without additives), and the other as the experimental (receives kefir with the addition of 0.5% lactulose syrup Lactusan).

Children aged 1 to 15 years were selected for the study, mainly with intestinal dysfunction, expressed in the form of constipation or irregularity of bowel movements with mucus, with pain in the abdomen. In some cases, the children also had diseases such as atopic dermatitis, infectious and others. The treatment was carried out on an outpatient basis, but the patients were under the constant supervision of doctors at Morozov Hospital. Each child received one package of the product daily. Thus, the daily dose was 200 g, and the course of the study lasted 20 days. Before and after the course, a bacteriological analysis of feces was performed.

RESEARCH RESULTS

This report presents data from the results of a study of 30 children who, in addition to clinical observation, had data on dysbacteriosis analysis before and after taking the course:

control group (kefir without lactulose) - 10 children,

experimental group (kefir with lactulose) - 20 children.

To evaluate the results, clinical data obtained by direct observation of patients by a doctor, as well as by interviewing parents of sick children and bacteriological analysis data were used.

In the control group, only 20% of children had an increase in the level of bifidobacteria, in the experimental group that took kefir with lactulose - 47%. In the change in the level of lactobacteria, the difference is even greater - 20% versus 65%, respectively. Regular kefir caused a decrease in lactobacteria in 50% of children, kefir with lactulose - only in 1 person (5%). At the same time, the stool in the experimental group normalized in all (100%) children.

General conclusion on clinical studies of kefir with lactulose, produced according to OST 49 29-84, amendment No. 7:

Kefir with lactulose, unlike regular kefir, has pronounced bifidogenic properties, that is, it significantly increases the number of bifidobacteria in the microbial pool of the large intestine.

Kefir with lactulose very noticeably increases the level of lactobacilli, which is not observed in the group that took regular kefir.

When taking kefir with lactulose, there is a suppression of pathogenic and conditionally pathogenic microflora.

Kefir with lactulose has a positive effect on intestinal function, eliminates constipation and other intestinal disorders in children.

The use of kefir with lactulose improves the clinical picture: reduces the manifestations of atopic dermatitis, improves appetite, improves the condition after an intestinal infection, improves the general condition of the body and intestinal function after antibiotic therapy.

CONCLUSIONS:

Kefir enriched with lactulose "Lactusan" according to OST 49 29-84, amendment No. 7, has a more favorable effect on the intestinal microflora than regular kefir.

We recommend kefir with lactulose (OST 49 29-84, amendment No. 7) as a mass product for the prevention of gastrointestinal diseases, including dysbacteriosis and intestinal dysfunction.

In the control group of 10 children, 7 (70%) had impaired intestinal function in the form of constipation. Of these, one had a stool with mucus.

After a course of kefir (without lactulose), the stool completely normalized in 5 children and partially in 2 children. increased in two children. decreased sharply in four children. Other changes in the composition of the microflora in the control group were rare and insignificant.

In the second group, where kefir was taken with a 0.5% lactulose supplement "Lactusan", 20 children aged 1 to 12 years. In 9 children (47%) in the experimental group, the level of bifidoflora after a course of kefir with lactulose "Lactusan" increased, but in three children (15%), who had viral and other diseases during the intake, it decreased by 1 log unit. An increase in the level of lactobacteria was observed in 13 children (65%), and three (15%) observed a slight decrease. Against the background of taking kefir with lactulose, there was a noticeable cleansing of the intestines from conditionally pathogenic flora (hemolytic, weakly fermenting and other forms, as well as Proteus, Klebsiella, staphylococci and others).

G.V., 1 year old, had dysbacteriosis of the 2nd degree and with low levels of lacto- and bifidobacteria, had a noticeably high level of Klebsiella (lg KOE = 6). This child was treated with phage, but there was no improvement. A course of treatment with kefir with "Lactusan" led to the disappearance of Klebsiella and an increase in the number of bifido- and lactoflora.

Significant improvements were seen in the clinic, when intestinal dysfunction disappeared and bowel movements normalized. In two other cases, when children had a reduced level of bifido- and lactoflora before treatment (K.K., 7 years old and Ch.M., 7 years old), complete normalization of microflora was achieved, and the tendency to constipation disappeared and bowel movements normalized.

In some cases, the effect of kefir was insufficient and an additional course of kefir with lactulose "Lactusan" was required.

In the child K.K., 7 years old, kefir helped eliminate constipation, but its effect on the composition of microflora was not sufficiently effective, because lactobacteria remained at a very low level. 6.

In another child (M.E., 3.5 years old), a course of kefir led to constipation, and this child was transferred to the experimental group, which received kefir with lactulose Lactusan. Normalization of stools and improvement of the composition of microflora were achieved.

Three children had atopic dermatitis. After a course of kefir with lactulose, its manifestations decreased sharply. In one child (P.D., 6 years old) after a course of kefir with "Lactusan" there was an increase in the level of proteus (up to lg KOE = 8), which is explained by the transfer at the end of the treatment of bronchitis and antibiotic treatment.

Similarly, in a child (A.A., 4 years old) due to food poisoning at the end of the course, the level of Escherichia increased by 100% (up to lg KOE = 8) due to hemolytic forms. However, in all of the listed cases, the general condition of the child improved, stools normalized, and the main indicators of bacterinary tests were at the level of normal values.

The beneficial effect of kefir with lactulose "Lactusan" compared to kefir without additives can be judged by the content of bifido- and lactobacteria in feces.

For a complete correctness of the comparison of the effect of kefir made using traditional technology and kefir enriched with lactulose, other things being equal, it is necessary to observe. For a correct comparison, the statistics did not take into account the data of bacterin analyses of children who had diseases (ARI, food poisoning, etc.) while taking the products whose effect is being studied, since it was the diseases that had been transferred that could have made adverse adjustments to the composition of the microflora.

If in the control group the increase in the level of bifidobacteria was only in 20% of children, then in the experimental group that took kefir with lactulose, it was in 47%. In the change in the level of lactobacteria, the difference is even greater - 20% versus 65%, respectively. children a decrease in lactobacteria, which sometimes exceeded 100-fold, kefir and with lactulose - in only 1 person (5%).